2WRV
Semi-synthetic highly active analogue of human insulin NMeHisB26-DTI- NH2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-19 |
Detector | ADSC QUANTUM 4r |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 39.170, 39.170, 123.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.340 - 2.150 |
R-factor | 0.20298 |
Rwork | 0.200 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mso |
RMSD bond length | 0.021 |
RMSD bond angle | 1.677 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.340 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.070 | 0.269 |
Number of reflections | 4113 | |
<I/σ(I)> | 14.5 | 6.8 |
Completeness [%] | 97.8 | 97.4 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | 0.1 NA CITRATE, 0.3 M TRIS PH 8.2, 0.6 MM ZN ACETATE, 0.06% PHENOL |