2WRV
Semi-synthetic highly active analogue of human insulin NMeHisB26-DTI- NH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 39.170, 39.170, 123.593 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.340 - 2.150 |
| R-factor | 0.20298 |
| Rwork | 0.200 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.677 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0082) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.340 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.070 | 0.269 |
| Number of reflections | 4113 | |
| <I/σ(I)> | 14.5 | 6.8 |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 0.1 NA CITRATE, 0.3 M TRIS PH 8.2, 0.6 MM ZN ACETATE, 0.06% PHENOL |
