2WQZ
Crystal structure of synaptic protein neuroligin-4 in complex with neurexin-beta 1: alternative refinement
Replaces: 2VH8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 158.520, 198.670, 85.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.900 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3BE8 AND 1C4R |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.000 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 25251 | |
| <I/σ(I)> | 16.1 | 3.3 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 8% PEG 20000, 0.1M MES PH6.5 |
