2WQT
Dodecahedral assembly of MhpD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-01 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 207.350, 207.350, 545.471 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.260 - 2.800 |
| R-factor | 0.22253 |
| Rwork | 0.222 |
| R-free | 0.23382 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sv6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.530 |
| Number of reflections | 210704 | |
| <I/σ(I)> | 6.7 | 1.8 |
| Completeness [%] | 97.9 | 95.7 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.2 | 1M NA/K PHOSPHATE PH3.2 |
