2WQS
Crystal structure of the C-terminal domain of Streptococcus gordonii surface protein SspB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.198, 61.011, 74.040 |
| Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
| Resolution | 36.623 - 1.700 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.220 |
| Number of reflections | 33858 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 94.4 | 94.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M MGCL2, 0.1 M TRIS-HCL, 30%(W/V) PEG4000, pH 8.5 |
