2WQD
Crystal structure of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 98.340, 98.340, 105.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.906 - 2.400 |
| R-factor | 0.2331 |
| Rwork | 0.228 |
| R-free | 0.29510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.910 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.460 |
| Number of reflections | 23409 | |
| <I/σ(I)> | 29.6 | 6.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M KCL 20% (W/V) PEG 3350 |
