2WQ3
GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride and nitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 56.670, 56.670, 56.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.140 - 1.220 |
| R-factor | 0.14351 |
| Rwork | 0.141 |
| R-free | 0.19989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wq1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.300 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.040 | 0.630 |
| Number of reflections | 9094 | |
| <I/σ(I)> | 17.6 | 2.01 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 3.57 | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25,5% (W/V) PEG 4000, 15% (V/V) GLYCEROL, 170MM NA-ACETATE, 90MM TRIS PH 8.5 |
