2WQ3
GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride and nitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | I 21 3 |
Unit cell lengths | 56.670, 56.670, 56.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.140 - 1.220 |
R-factor | 0.14351 |
Rwork | 0.141 |
R-free | 0.19989 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wq1 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 1.300 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.040 | 0.630 |
Number of reflections | 9094 | |
<I/σ(I)> | 17.6 | 2.01 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 3.57 | 3.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25,5% (W/V) PEG 4000, 15% (V/V) GLYCEROL, 170MM NA-ACETATE, 90MM TRIS PH 8.5 |