2WQ1
GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 56.580, 56.580, 56.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.330 - 1.080 |
| R-factor | 0.12938 |
| Rwork | 0.127 |
| R-free | 0.16748 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.834 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.150 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.080 | 0.660 |
| Number of reflections | 13026 | |
| <I/σ(I)> | 10 | 2.01 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 4.11 | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG 3350, 200 MM NABR, 100 MM BIS-TRIS PROPANE PH 8.5 |
