2WQ0
GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 56.440, 56.440, 56.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.850 - 1.120 |
| R-factor | 0.15069 |
| Rwork | 0.148 |
| R-free | 0.19718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wq1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.624 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.190 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.040 | 0.660 |
| Number of reflections | 11605 | |
| <I/σ(I)> | 18.8 | 2.2 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 4.74 | 4.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.4 M (NH4)2HPO4, 100 MM TRIS PH 8.5 |
