2WPW
Tandem GNAT protein from the clavulanic acid biosynthesis pathway (without AcCoA)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.449, 93.501, 126.430 |
| Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
| Resolution | 32.400 - 2.380 |
| R-factor | 0.21151 |
| Rwork | 0.209 |
| R-free | 0.26563 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.134 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.130 | 0.350 |
| Number of reflections | 68408 | |
| <I/σ(I)> | 6.5 | 3 |
| Completeness [%] | 92.2 | 71.9 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 3 MG/ML PROTEIN, 10% PEG8000, 0.1M CACODYLATE PH6, 0.2M SODIUM ACETATE |
