2WPV
Crystal structure of S. cerevisiae Get4-Get5 complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.275, 118.770, 168.377 |
| Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
| Resolution | 25.590 - 1.990 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.20600 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE-RESOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.070 | 0.330 |
| Number of reflections | 239510 | |
| <I/σ(I)> | 15.3 | 3.7 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM DI-AMMONIUM HYDROGEN CITRATE AND 20% (V/V) PEG3350 |
