2WPS
Salmonella enterica SadA 483-523 fused to GCN4 adaptors (SadAK3b-V2, out-of-register fusion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.660, 55.850, 168.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.830 - 2.600 |
| R-factor | 0.24193 |
| Rwork | 0.238 |
| R-free | 0.32443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.550 |
| Number of reflections | 11193 | |
| <I/σ(I)> | 7.61 | 2.01 |
| Completeness [%] | 98.7 | 97.1 |
| Redundancy | 3.47 | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% (W/V) PEG 4000, 10% (V/V) ISOPOPANOL, 3% (W/V) 1,5-DIAMINOPENTANE DIHYDROCHLORIDE, 100 MM NA-CITRATE PH 5.6 |
