2WPL
factor IXa superactive triple mutant, EDTA-soaked
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.420, 66.710, 98.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.580 - 1.820 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wph |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.570 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 1.910 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.070 | 0.640 |
| Number of reflections | 22189 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 82.4 | 71.4 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 22 % PEG 3000 100 MM BIS/TRIS PH 6.85 |
