2WP9

Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhB His207Thr mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-24
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	119.853, 183.803, 202.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.850 - 2.700
R-factor	0.19192
Rwork	0.190
R-free	0.22247
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wdq
RMSD bond length	0.012
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.630	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.640
Number of reflections	122882
<I/σ(I)>	11.1	2
Completeness [%]	99.9	100
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.1M TRIS PH 8.5, 0.1M LI2SO4, 0.1M NACL, 0.009% DDM, 3% 1,6-HEXANEDIOL AND 10% (W/V) PEG4000