2WP0
Crystal structure of a HobA-DnaA (domain I-II) complex from Helicobacter pylori.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-17 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.560, 55.500, 96.830 |
| Unit cell angles | 90.00, 97.55, 90.00 |
Refinement procedure
| Resolution | 50.756 - 2.670 |
| R-factor | 0.2283 |
| Rwork | 0.226 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uvp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.506 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.770 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.120 | 0.550 |
| Number of reflections | 17498 | |
| <I/σ(I)> | 6.8 | 1.7 |
| Completeness [%] | 93.4 | 94.7 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS-HCL PH 8.0, 200 MM K-ACETATE, 19-22% PEG 3350 |
