2WOO
Nucleotide-free form of S. pombe Get3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.795, 92.922, 286.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.687 - 3.006 |
R-factor | 0.2397 |
Rwork | 0.237 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MONOMER OF PDB ENTRY 2WOJ |
RMSD bond length | 0.008 |
RMSD bond angle | 1.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.060 | 0.620 |
Number of reflections | 37723 | |
<I/σ(I)> | 24.2 | 1.7 |
Completeness [%] | 94.2 | 81.4 |
Redundancy | 4.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG 3350, 0.1 M MES PH 6.5, 0.4 M MGCL2 |