2WOO
Nucleotide-free form of S. pombe Get3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.795, 92.922, 286.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.687 - 3.006 |
| R-factor | 0.2397 |
| Rwork | 0.237 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MONOMER OF PDB ENTRY 2WOJ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 37723 | |
| <I/σ(I)> | 24.2 | 1.7 |
| Completeness [%] | 94.2 | 81.4 |
| Redundancy | 4.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG 3350, 0.1 M MES PH 6.5, 0.4 M MGCL2 |
