2WNY
Structure of Mth689, a DUF54 protein from Methanothermobacter thermautotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.576, 61.021, 88.311 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.230 - 1.950 |
| R-factor | 0.19227 |
| Rwork | 0.191 |
| R-free | 0.24705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ogk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.283 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 24137 | |
| <I/σ(I)> | 18.3 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M LISO4, 0.1M HEPES PH 7.5, 25% PEG 3350 |
