2WNX
3b' carbohydrate-binding module from the Cel9V glycoside hydrolase from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.129, 74.129, 82.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.310 |
| R-factor | 0.12127 |
| Rwork | 0.120 |
| R-free | 0.15439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbc |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.044 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 57111 | |
| <I/σ(I)> | 48.1 | 1.86 |
| Completeness [%] | 94.6 | 81.5 |
| Redundancy | 8.1 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.2 M AMMONIUM ACETATE, 0.1 M TRI-SODIUM CITRATE DIHYDRATE PH 5.6, 30% W/V POLYETHYLENE GLYCOL 4000 |
