2WNO
X-ray Structure of CUB_C domain from TSG-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-01 |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.929, 56.929, 112.572 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.260 - 2.300 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL OF TARGETS BASED UPON 1SZB |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.300 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 8616 | |
| <I/σ(I)> | 8.5 | 1.3 |
| Completeness [%] | 98.7 | 92.1 |
| Redundancy | 5.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH 7.5 200MM MGSO4 22% (W/V) PEG 1000 |
