2WNF
Crystal Structure of a Mammalian Sialyltransferase in complex with Gal-beta-1-3GalNAc-ortho-nitrophenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2008-09-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.987, 78.347, 99.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.110 - 1.250 |
| R-factor | 0.18115 |
| Rwork | 0.180 |
| R-free | 0.19529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wml |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.110 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 85229 | |
| <I/σ(I)> | 23.4 | 2.8 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 6.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000 AND 0.1 M MES, PH 6.8 |
