2WNB
Crystal Structure of a Mammalian Sialyltransferase in complex with disaccharide and CMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-09-09 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.987, 78.347, 99.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.340 - 1.550 |
| R-factor | 0.17728 |
| Rwork | 0.175 |
| R-free | 0.21339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wml |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.313 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 52716 | |
| <I/σ(I)> | 19.3 | 3.5 |
| Completeness [%] | 85.0 | 83 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 4000 AND 0.1 M MES, PH 6.8 |
