2WN3
Crystal structure of Discoidin I from Dictyostelium discoideum in complex with the disaccharide GalNAc beta 1-3 galactose, at 1.6 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.580, 274.000, 85.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 137.000 - 1.590 |
| R-factor | 0.15301 |
| Rwork | 0.152 |
| R-free | 0.18023 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w94 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.577 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.730 | 1.670 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 114648 | |
| <I/σ(I)> | 9.8 | 2.6 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH7.5, LI2SO4 1.2M, 20% GLYCEROL AS CRYOPROTECTANT |
