2WN2
Structure of the discoidin I from Dictyostelium discoideum in complex with galactose beta 1-3 galNAc at 1.8 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.470, 273.700, 85.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.330 - 1.820 |
| R-factor | 0.143 |
| Rwork | 0.141 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w94 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.597 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.090 | 0.380 |
| Number of reflections | 76712 | |
| <I/σ(I)> | 15.1 | 4.7 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH 7.5, LI2SO4 1.2M, 20% GLYCEROL AS CRYOPROTECTANT |
