2WMN
Structure of the complex between DOCK9 and Cdc42-GDP.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-18 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.050, 88.472, 90.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.454 - 2.391 |
R-factor | 0.2225 |
Rwork | 0.220 |
R-free | 0.27620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wm9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.120 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.440 |
Number of reflections | 28789 | |
<I/σ(I)> | 10 | 2.5 |
Completeness [%] | 96.4 | 94.3 |
Redundancy | 4.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | pH 6.0 |