2WM9

Structure of the complex between DOCK9 and Cdc42.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-30
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	88.410, 93.980, 88.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.463 - 2.200
R-factor	0.2114
Rwork	0.209
R-free	0.25110
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.012
RMSD bond angle	1.325
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.520
Number of reflections	37540
<I/σ(I)>	10.9	2.8
Completeness [%]	99.1	96.2
Redundancy	3.6	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	THE PROTEIN WAS CONCENTRATED TO 6 MG/ML. CRYSTALS WERE GROWN USING HANGING DROP VAPOUR DIFFUSION. 1 UL OF COMPLEX WITH 200 MM NDSB-201 ADDED TO PROTEIN PRIOR TO CRYSTALLIZATION WAS MIXED WITH EQUAL VOLUME OF CRYSTALLIZATION BUFFER: 14% (W/V) PEG3350,100 MM MES (PH 6.0), 200 MM AMMONIUM ACETATE, 12% GLYCEROL. CRYSTALS WERE GROWN AT 20C.