2WLZ
Chitinase A from Serratia marcescens ATCC990 in complex with Chitobio- thiazoline.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 130.643, 201.272, 59.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.760 - 1.820 |
| R-factor | 0.18581 |
| Rwork | 0.184 |
| R-free | 0.21778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.737 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0097) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.170 | 1.060 |
| Number of reflections | 69869 | |
| <I/σ(I)> | 7.4 | 0.8 |
| Completeness [%] | 97.3 | 81.3 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.0M SODIUM CITRATE 10MM SODIUM BORATE PH8 DIOXANE 20%, pH 8.0 |
