2WLX
Putative thiosulfate sulfurtransferase YnjE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-17 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.400, 83.430, 131.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.770 - 1.900 |
| R-factor | 0.19886 |
| Rwork | 0.197 |
| R-free | 0.25056 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.742 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 219109 | |
| <I/σ(I)> | 13.4 | 4.5 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 291 | 20% PEG 10000, 0.1M HEPES PH 7.5, TEMP 18 C |
