2WLO
POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-10 |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.860, 115.360, 289.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.995 - 4.036 |
R-factor | 0.2453 |
Rwork | 0.245 |
R-free | 0.25470 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 1.020 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.000 |
Rmerge | 0.224 |
Number of reflections | 0 |
<I/σ(I)> | 9.5 |
Completeness [%] | 98.4 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PRECIPITANT: 15 % PEG400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN KIRBAC 3.1, 8MG/ML 150MM KCL 20MM TRIS PH 8.0 20MM TETRABUTYL AMMONIUM BROMIDE 4MM CYMAL-5 0.05% TDM PROTEIN AND PRECIPITANT WERE MIXED TOGETHER IN A 1:1 RATIO, AND EQUILIBRATED AGAINST RESERVOIR SOLUTION IN A SITTING-DROP VAPOUR-DIFFUSION SET-UP. |