2WLN

POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-31
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	106.680, 109.060, 146.390
Unit cell angles	73.95, 82.30, 89.88

Refinement procedure

Resolution	15.000 - 3.440
R-factor	0.2664
Rwork	0.266
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xl4
RMSD bond length	0.019
RMSD bond angle	1.671
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	3.790
High resolution limit [Å]	3.600	3.600
Rmerge	0.180	0.860
Number of reflections	94851
<I/σ(I)>	4.1	1.6
Completeness [%]	97.0	97.1
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		PRECIPITANT: 15 % PEG 400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN: KIRBAC3.1, 8MG/ML 150MM KCL 20MM TRIS PH 8.0 0.05% TDM 10MM EDTA 4MM LDAO PROTEIN AND PRECIPITANT WERE MIXED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT SOLUTION IN A SITTING DROP VAPOUR DIFFUSION SET-UP.