2WLM
POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-10 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 99.261, 151.193, 294.757 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.971 - 3.610 |
R-factor | 0.2645 |
Rwork | 0.263 |
R-free | 0.28810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xl4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.130 | 0.460 |
Number of reflections | 25733 | |
<I/σ(I)> | 12 | 3.8 |
Completeness [%] | 94.7 | 92 |
Redundancy | 6.3 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | PRECIPITANT 40 % PEG 200 0.2 M SODIUM CHLORIDE 0.1 M SODIUM PHOSPHATE PH 6.0 PROTEIN KIRBAC3.1, 8MG/ML 150MM KCL 20MM BACL2 4MM LDAO 0.05% TDM 0.2MM PI(4,5)P2 PROTEIN AND PRECIPITANT WERE COMBINED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT IN A SITTING DROP VAPOUR DIFFUSION SET UP. |