2WLM

POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-10
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	99.261, 151.193, 294.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.971 - 3.610
R-factor	0.2645
Rwork	0.263
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xl4
RMSD bond length	0.006
RMSD bond angle	1.035
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.730
High resolution limit [Å]	3.600	3.600
Rmerge	0.130	0.460
Number of reflections	25733
<I/σ(I)>	12	3.8
Completeness [%]	94.7	92
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		PRECIPITANT 40 % PEG 200 0.2 M SODIUM CHLORIDE 0.1 M SODIUM PHOSPHATE PH 6.0 PROTEIN KIRBAC3.1, 8MG/ML 150MM KCL 20MM BACL2 4MM LDAO 0.05% TDM 0.2MM PI(4,5)P2 PROTEIN AND PRECIPITANT WERE COMBINED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT IN A SITTING DROP VAPOUR DIFFUSION SET UP.