2WLI

POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-18
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	113.409, 105.009, 89.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.090
R-factor	0.26655
Rwork	0.265
R-free	0.28767
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xl4
RMSD bond length	0.012
RMSD bond angle	1.339
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.130	0.640
Number of reflections	20089
<I/σ(I)>	16.6	3.5
Completeness [%]	100.0	100
Redundancy	7.9	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		PRECIPITANT: 15 % PEG 400 2.5 % PEG 4000 2.5 % PEG 8000 10 % GLYCEROL 90 MM HEPES PH 7.5 PROTEIN: 8MG/ML KIRBAC3.1 150MM KCL 20MM TRIS8.0 0.05% TDM 4MM LDAO PROTEIN AND PRECIPITANT WERE COMBINED IN A 1:1 RATIO AND EQUILIBRATED AGAINST A RESERVOIR OF PRECIPITANT IN A SITTING-DROP VAPOUR-DIFFUSION SET-UP.