2WLH

POTASSIUM CHANNEL FROM MAGNETOSPIRILLUM MAGNETOTACTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-21
Detector	ADSC CCD
Spacegroup name	P 4 21 2
Unit cell lengths	106.279, 106.279, 90.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.280
R-factor	0.216
Rwork	0.213
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xl4
RMSD bond length	0.013
RMSD bond angle	1.329
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.400
High resolution limit [Å]	3.280	3.280
Rmerge	0.110	0.520
Number of reflections	8448
<I/σ(I)>	18.8	3
Completeness [%]	97.6	90.4
Redundancy	9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		PRECIPITANT: 40 % PEG 200 0.2 M SODIUM CHLORIDE 0.1 M SODIUM PHOSPHATE PH 6.0 PROTEIN: 8MG/ML KB3.1 150MM KCL 20MM TRIS PH 8.0 0.05% TDM 4MM LDAO PROTEIN PRECIPITANT COMBINED IN 1:1 RATIO, THEN EQUILIBRATED IN SITTING-DROP VAPOUR-DIFFUSION SET-UP WITH RESERVOIR OF PRECIPITANT.