2WL7
Crystal structure of the PSD93 PDZ1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-28 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 85.198, 85.198, 45.515 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.747 - 2.028 |
| R-factor | 0.1687 |
| Rwork | 0.164 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i1n |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.703 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.670 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.070 | 0.310 |
| Number of reflections | 7993 | |
| <I/σ(I)> | 27.2 | 8.2 |
| Completeness [%] | 97.9 | 85.8 |
| Redundancy | 11.2 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.01 M NICL2, 0.1 M TRIS, PH 8.5, 1 M LI2SO4 |
