2WKG
Nostoc punctiforme Debranching Enzyme (NPDE)(Native form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 97 |
Detector technology | CCD |
Collection date | 2008-03-16 |
Detector | ADSC CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 93.731, 93.731, 257.042 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.880 - 3.000 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1joj |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.880 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.310 |
Number of reflections | 13517 | |
<I/σ(I)> | 11.3 | 12.8 |
Completeness [%] | 95.6 | 100 |
Redundancy | 22.6 | 23.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 20% POLYETHYLENE GLYCOL 8000, 200MM TRIS CL (PH8.5),100MM MGCL2 |