2WKF
Crystal Structure of Macrophage Migration Inhibitory Factor from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-07-24 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 83.291, 83.291, 83.291 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.900 - 2.050 |
| R-factor | 0.253 |
| Rwork | 0.249 |
| R-free | 0.32057 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wkb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.040 | 0.390 |
| Number of reflections | 12419 | |
| <I/σ(I)> | 34.6 | 6.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.2 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10% PEG 1000, 10% PEG 8000, 0.2 M KSCN, 0.1 M TRIS-HCL PH 8.0 |
