2WKD

Crystal structure of a double Ile-to-Met mutant of protein ORF34 from lactococcus phage p2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-30
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	75.740, 75.740, 88.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.020 - 2.100
R-factor	0.24
Rwork	0.239
R-free	0.25620
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.186
Data scaling software	TRUNCATE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.040	2.120
High resolution limit [Å]	2.100	2.100
Rmerge	0.050	0.440
Number of reflections	9205
<I/σ(I)>	25.79	3.77
Completeness [%]	99.8	100
Redundancy	10.18	10.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2		PROTEIN AT 7 MG/ML IN 10 MM TRIS, 300 MM NACL, 1 MM TCEP, PH 8.0 WAS CRYSTALLIZED BY SITTING-DROP VAPOUR DIFFUSION AGAINST 0.1 M HEPES PH 7.2, 60% MME-PEG550