2WKC
Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-10 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.280, 71.710, 109.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.028 - 2.600 |
R-factor | 0.197 |
Rwork | 0.192 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wkd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.032 |
Data scaling software | TRUNCATE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.620 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.400 |
Number of reflections | 12049 | |
<I/σ(I)> | 11.72 | 3.03 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 3.54 | 3.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | PROTEIN AT 7 MG/ML IN 10 MM TRIS, 300 MM NACL PH 8.0 WAS CRYSTALLIZED BY SITTING-DROP VAPOUR DIFFUSION AGAINST 0.1 M SODIUM CACODYLATE PH 6.2, 45% MME-PEG2000 |