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2WKC

Crystal structure from a single-stranded DNA binding protein from the lactococcal phage p2

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID23-2
Synchrotron siteESRF
BeamlineID23-2
Temperature [K]100
Detector technologyCCD
Collection date2008-05-10
DetectorMARMOSAIC 225 mm CCD
Spacegroup nameP 21 21 21
Unit cell lengths48.280, 71.710, 109.730
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution60.028 - 2.600
R-factor0.197
Rwork0.192
R-free0.25090
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2wkd
RMSD bond length0.008
RMSD bond angle1.032
Data scaling softwareTRUNCATE
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0088)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0002.620
High resolution limit [Å]2.6002.600
Rmerge0.1100.400
Number of reflections12049
<I/σ(I)>11.723.03
Completeness [%]98.899.8
Redundancy3.543.55
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.2PROTEIN AT 7 MG/ML IN 10 MM TRIS, 300 MM NACL PH 8.0 WAS CRYSTALLIZED BY SITTING-DROP VAPOUR DIFFUSION AGAINST 0.1 M SODIUM CACODYLATE PH 6.2, 45% MME-PEG2000

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