2WKB
Crystal Structure of Macrophage Migration Inhibitory Factor from Plasmodium berghei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-08-31 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.703, 69.169, 80.217 |
| Unit cell angles | 90.00, 118.20, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.780 |
| R-factor | 0.2019 |
| Rwork | 0.199 |
| R-free | 0.25455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2os5 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.039 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 72962 | |
| <I/σ(I)> | 17.35 | 1.36 |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 13% PEG 6000, 0.2 M KSCN |
