2WK4
Dimeric structure of D347G D348G mutant of the sapporovirus RNA dependent RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MAR555 FLAT PANEL |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 132.613, 132.613, 150.582 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.010 - 2.980 |
R-factor | 0.20309 |
Rwork | 0.198 |
R-free | 0.29208 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ckw |
RMSD bond length | 0.013 |
RMSD bond angle | 1.609 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.210 | 0.990 |
Number of reflections | 29506 | |
<I/σ(I)> | 10.8 | 2 |
Completeness [%] | 96.0 | 72.8 |
Redundancy | 7.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |