2WK4
Dimeric structure of D347G D348G mutant of the sapporovirus RNA dependent RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 132.613, 132.613, 150.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.010 - 2.980 |
| R-factor | 0.20309 |
| Rwork | 0.198 |
| R-free | 0.29208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ckw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.609 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.210 | 0.990 |
| Number of reflections | 29506 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 96.0 | 72.8 |
| Redundancy | 7.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
