2WJW
Crystal structure of the human ionotropic glutamate receptor GluR2 ATD region at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.977, 122.455, 138.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.161 - 1.800 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.862 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.700 |
| Number of reflections | 40760 | |
| <I/σ(I)> | 23.6 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.08M SODIUM ACETATE, PH 4.6 0.16M AMMONIUM SULPHATE 20 (W/V) PEG4000 20% (V/V) GLYCEROL |
