2WJG

Structure and function of the FeoB G-domain from Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	84.770, 84.770, 137.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.287 - 2.200
R-factor	0.2177
Rwork	0.216
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.215
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.200	2.230
Rmerge	0.090	0.780
Number of reflections	24483
<I/σ(I)>	29.2	3.5
Completeness [%]	93.3	89.3
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1