2WJD
Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.850, 88.850, 92.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.076 - 2.799 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.23190 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.931 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.180 |
| Number of reflections | 9531 | |
| <I/σ(I)> | 58.9 | 18.2 |
| Completeness [%] | 99.3 | 97.3 |
| Redundancy | 22.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M (NH4)2SO4, 0.1 M TRIS-CL PH 8.5 |
