2WJ7
human alphaB crystallin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-30 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 49.460, 66.070, 190.970 |
Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
Resolution | 39.034 - 2.631 |
R-factor | 0.2553 |
Rwork | 0.252 |
R-free | 0.32000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wj5 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.071 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 190.000 | 2.770 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.220 | 0.630 |
Number of reflections | 17862 | |
<I/σ(I)> | 9.6 | 1.9 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 4 DAYS AT 16 DEGREESC IN 100 MM BICINE PH 9.0, 55% 2-METHYL-2,4-PENTANDIOL, AT A PROTEIN CONCENTRATION OF 20 MG/ML USING 1 UL PROTEIN SOLUTION AND 2 UL RESERVOIR SOLUTION. |