2WJ5
Rat alpha crystallin domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-05 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.479, 37.964, 28.081 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 28.071 - 1.120 |
R-factor | 0.1537 |
Rwork | 0.152 |
R-free | 0.18020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gme |
RMSD bond length | 0.025 |
RMSD bond angle | 2.530 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.150 |
High resolution limit [Å] | 1.120 | 1.120 |
Rmerge | 0.090 | 0.400 |
Number of reflections | 39225 | |
<I/σ(I)> | 10.7 | 2.5 |
Completeness [%] | 91.6 | 51.5 |
Redundancy | 6.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 289 | SINGLE CRYSTALS OF RAT HSP20 ACD GREW AT 16 DEGREESIN 100 MM MES PH 6.5, BETWEEN 45-52% V/V PEG 200, AT A PROTEIN CONCENTRATION OF AROUND 10 MG/ML USING 1 UL PROTEIN SOLUTION AND 2 UL RESERVOIR SOLUTION. |