2WIU
Mercury-modified bacterial persistence regulator hipBA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 166.933, 166.933, 124.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 166.670 - 2.350 |
| R-factor | 0.21843 |
| Rwork | 0.216 |
| R-free | 0.26389 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.030 | 0.320 |
| Number of reflections | 73235 | |
| <I/σ(I)> | 23.26 | 3.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 8.8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2M AMMONIUM SULFATE, HEPES PH 7.6, 6% ETHYLENE GLYCOL 10 MG/ML PROTEIN |
