2WIU
Mercury-modified bacterial persistence regulator hipBA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-06 |
Detector | ADSC CCD |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 166.933, 166.933, 124.577 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 166.670 - 2.350 |
R-factor | 0.21843 |
Rwork | 0.216 |
R-free | 0.26389 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.018 |
RMSD bond angle | 2.077 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.450 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.030 | 0.320 |
Number of reflections | 73235 | |
<I/σ(I)> | 23.26 | 3.9 |
Completeness [%] | 99.5 | 100 |
Redundancy | 8.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 2M AMMONIUM SULFATE, HEPES PH 7.6, 6% ETHYLENE GLYCOL 10 MG/ML PROTEIN |