2WIS
Fluorescent protein KillerRed in the bleached state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.940, 73.400, 75.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.350 |
| R-factor | 0.226 |
| Rwork | 0.222 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wiq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.410 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 17145 | |
| <I/σ(I)> | 20.8 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 1.8M AMMONIUM SULFATE 0.1M TRIS PH 8.5, 0.05M ASCORBIC ACID, VAPOR DIFFUSION, TEMPERATURE 293K |
