2WIQ
Fluorescent protein KillerRed in the native state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-28 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.490, 73.670, 80.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.310 - 2.000 |
R-factor | 0.17382 |
Rwork | 0.171 |
R-free | 0.23564 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gfl |
RMSD bond length | 0.018 |
RMSD bond angle | 1.882 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.150 | 0.580 |
Number of reflections | 29194 | |
<I/σ(I)> | 14.7 | 3.3 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 1.8M AMMONIUM SULFATE, 0.1M TRIS PH 8.5, 0.05M ASCORBIC ACID, VAPOR DIFFUSION, TEMPERATURE 293K |