2WIC
Crystal Structures of the N-terminal Intracellular Domain of FeoB from Klebsiella Pneumoniae in GMPPNP binding state
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Wavelength(s) | 0.97869 , 0.97888, 0.96365 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 106.897, 106.897, 106.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.930 - 2.050 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.300 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.930 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 48292 | |
| <I/σ(I)> | 22.7 | 3.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS-HCL PH 8.5, 25% PEG4000, 100 MM NA ACETATE |
