2WI8
Crystal structure of the triscatecholate siderophore binding protein FeuA from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.710, 54.710, 177.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.230 - 1.550 |
| R-factor | 0.18267 |
| Rwork | 0.182 |
| R-free | 0.21544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.260 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.370 |
| Number of reflections | 39175 | |
| <I/σ(I)> | 17.5 | 4.8 |
| Completeness [%] | 98.1 | 95.9 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN WAS CRYSTALLIZED FROM 30% (V/V) JEFFAMINE ED-2001 PH 7.0, 100 MM HEPES, PH 8.0; THEN SOAKED IN MOTHER LIQUOR CONTAINING 30% (V/V) GLYCEROL FOR CRYOPROTECTION |
