2WI8
Crystal structure of the triscatecholate siderophore binding protein FeuA from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-16 |
Detector | ADSC CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 54.710, 54.710, 177.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.230 - 1.550 |
R-factor | 0.18267 |
Rwork | 0.182 |
R-free | 0.21544 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2phz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.260 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 39175 | |
<I/σ(I)> | 17.5 | 4.8 |
Completeness [%] | 98.1 | 95.9 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | PROTEIN WAS CRYSTALLIZED FROM 30% (V/V) JEFFAMINE ED-2001 PH 7.0, 100 MM HEPES, PH 8.0; THEN SOAKED IN MOTHER LIQUOR CONTAINING 30% (V/V) GLYCEROL FOR CRYOPROTECTION |