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2WI8

Crystal structure of the triscatecholate siderophore binding protein FeuA from Bacillus subtilis

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyCCD
Collection date2008-02-16
DetectorADSC CCD
Spacegroup nameP 41 21 2
Unit cell lengths54.710, 54.710, 177.660
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.230 - 1.550
R-factor0.18267
Rwork0.182
R-free0.21544
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2phz
RMSD bond length0.012
RMSD bond angle1.349
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]19.2601.640
High resolution limit [Å]1.5501.550
Rmerge0.0600.370
Number of reflections39175
<I/σ(I)>17.54.8
Completeness [%]98.195.9
Redundancy4.74.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
18PROTEIN WAS CRYSTALLIZED FROM 30% (V/V) JEFFAMINE ED-2001 PH 7.0, 100 MM HEPES, PH 8.0; THEN SOAKED IN MOTHER LIQUOR CONTAINING 30% (V/V) GLYCEROL FOR CRYOPROTECTION

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