2WHN
N-terminal domain from the PilC type IV pilus biogenesis protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.168, 55.168, 152.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.050 |
| R-factor | 0.21899 |
| Rwork | 0.216 |
| R-free | 0.26994 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.521 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 14807 | |
| <I/σ(I)> | 18.7 | 3.2 |
| Completeness [%] | 95.4 | 86.9 |
| Redundancy | 11.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
