2WHM
Cellvibrio japonicus Man26A E121A and E320G double mutant in complex with mannobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 41 |
Unit cell lengths | 93.177, 93.177, 53.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 1.500 |
R-factor | 0.145 |
Rwork | 0.144 |
R-free | 0.16500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gw1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.056 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.270 |
Number of reflections | 73813 | |
<I/σ(I)> | 53 | 5.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 100MMTRIS PH7, 26% MEONOMETHYLETHER PEG550, 3MM ZNSO4, pH 7.5 |